RNA

Part:BBa_K199032:Design

Designed by: Olivia Ho-Shing   Group: iGEM09_MoWestern_Davidson   (2009-07-13)

pBad-CCAUC Suppressor tRNA (10-bp Anticodon)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 139
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 176
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 189


Design Notes

We used the pBad promoter to transcribe lower levels of tRNA in a high copy plasmid, pSB1A2.

Source

The pBad promoter is from the BioBrick Parts registry. Suppressor tRNA was made by de novo synthesis from single-stranded oligos. Based on the paper by Anderson et al. 2002.

References

[http://gcat.davidson.edu/GcatWiki/images/6/6f/AndersonSchultz2002ChemBiol.pdf (1) Anderson JC, Magliery TJ, Schultz PG. 2002. Exploring the limits of codon and anticodon size. Chemistry & Biology 9:234-44.]